The Phytochemical Investigation and Biological Activities of Berberis Orthobotrys

Akber Dad, Iftikhar Ali, Nadja Engel, Muhammad Atif, Hidayat Hussain, Viqar Uddin Ahmad, Peter Langer, Ivan R. Green



The leaf extract of Berberis orthobotrys Bien. ex Aitch. (B.o.) afforded three compounds viz., β-sitosterol (1), sesamin (2) and 10-eicosanol (3) which were identified by spectroscopic methods including 1D and 2D NMR, GC-MS, IR and comparison of their spectral data with the published data. To the best of our knowledge these three compounds are reported here for the first time to be isolated from Berberis orthobotrys Bien. ex Aitch. (B.o.). Moreover the root extract exhibited good antilieshmanial activity and root and fruit extracts demonstrated minor antifungal activity against Fusarium solani.


Berberis orthobotrys, Bioassays, Sesamin, 10-Eicosanol

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[1] Ali M, Malik AR, Sharma KR. Vegetative propagation of Berberis aristata DC. An endangered Himalayan shrub. J Med Plant Res 2008;2:374-377.

[2] Hill SR. Conservation assessment for American Berberry (Berberis canadensis Mill.). University of Illinois at Urbana, Champaign, Centre for Biodiversity. Technical Report 2003, (9).

[3] Alamgeer, Akhtar MS, Jabeen Q, Bashir S, Malik MNH, Karim S, Mushtaq MN, Rasool S, Latif F, Tabbasum N, Khan AQ, Ahsan H, Khan W, Javed I. Possible mechanism of cardiac depressant activity of Berberis orthobotrys roots in isolated rabbit heart. Acta Poloniae Pharmaceutica - Drug Research 2014;71:667-675.

[4] Alamgeer, Ghuffar A, Ahmad T, Mushtaq MN. Antihyperlipidemic effect of Berberis orthobotrys in hyperlipidemic animal models. Bangladesh J Pharmacol 2014;9:377-382.

[5] Khan SW, Khatoon S. Ethnobotanical studies on useful trees and shrubs of Haramosh and Bugrote valleys in Gilgit, Northern areas of Pakistan. Pak J Bot 2007;39: 699-710.

[6] Abbas Q, Khan SW, Khatoon S, Hussain SA, Hassan SN, Hussain A, Qureshi R, Hussain I. Floristic biodiversıty and traditional uses of medicinal plants of Haramosh valley, Central Karakoram National Park of Gilgit district, Gilgit-Baltistan. Pak J Bio Env Sci 2014;5:75-86.

[7] Noor A, Khatoon S, Ahmed M, Razaq A. Ethnobotanical study on some useful shrubs of Astore valley, Gilgit-Baltistan, Pakistan. Bangladesh J Bot 2014;43:19-25.

[8] Maliwichi-Nyirenda CP, Maliwichi LL, Franco M. Germination response of Threatened African medicinal Berbery (Berberis holstii) under light, stratification and temperature treatments. J Med Plant Res 2011;6:88-93.

[9] Alamgeer, Akhtar MS, Jabeen Q, Akram M, Khan HU, Karim S, Malik MNH, Mushtaq MN, Salma U. Antihypertensive activity of aqueous-methanol extract of Berberis orthobotrys. Trop J Pharm Res 2013;12:393-399.

[10] Gulfraz M, Asad MJ, Qaddir G, Mehmood S, Shaukat S, Perveen Z. Phytochemical Constituents of Berberis lyceum Royle and Justicia adhatoda. J Chem Soc Pak 2008;30:453-457.

[11] Hussain SF, Khan L, Sadozai KK, Shamma M. New alkaloids from Berberis orthobotrys. J Nat Prod 1981;44:274-278.

[12] Zaidi SH. Existing indigenous medicinal plant resources of Pakistan and their prospects for utilization. Pak For J 1998;48:1-5.

[13] McLaughlin JL. Brine shrimp, crown gall tumors: simple bioassays for the discovery of plant antitumor agents. Proceedings of NIH Workshop, Bioassay for Discovery of Antitumoral, Antiviral Agents from Natural Sources, Bethesda. 1988, p. 22.

[14] Meyer BN, Ferrigni NR, Putnam JE, Jacobson LB, Nichols E, McLaughlin JL. Brine shrim: A convenient general bioassay for active plant constituents. Planta Med 1982;45:31-34.

[15] Finney DJ. Probit Analysis, 3rd ed., Cambridge University Press, Cambridge, 1971 p. 333.

[16] Ali I, Rubina N. Wahib NK, Rukhsana G, Choudhary, MI. Biological screening of different root extracts of Euphorbra wallichia. Pak. J. Bot. 2009;41:1737-1741.

[17] Kavanagh F. Analytical Microbiology. Academic Press London. 1963, 125-141. [18] Carron RA, Maran JM, Montero-Fernandozaigo L, Dominguez AA. Plantes Medicinales et Phytotherapic 1987;21:195-202.


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