In vitro cytotoxicity studies of sixteen plants used for pregnant women’s health conditions in Menoua Division-West Cameroon

Didiane Mefokou Yemele, Bruno Phelix Telefo, Stephanie Chekem Goka, Benoit Telesphore Nguelefack, Pierre Simeon chegaing Fodouop, Richard Simo Tagne, Landry Lienou Lienou, Filomain Nguemo, Paul Fewou Moundipa

Abstract


In Cameroon, many plants are used in traditional medicine for the treatment of pregnancy and childbirth complaints. However, toxicological potential of most of these plants have not been investigated. In order to evaluate the degree of safety of their users, in vitro cytotoxic potentials of sixteen of these medicinal plants were subjected to the assay using the brine shrimp lethality assay. From this study, the aqueous extract of plant Rauvolfia vomitoria bark was found to be cytotoxic and that of Ageratum conyzoides stem and leaves slightly cytotoxic, with LC50 values of 17.62 and 99.17µg/ml, respectively. The least toxic plant extracts were Aloe buttneri, Commelina benghalensis, Ipomoea tenuirostrisandNelsonia canescens, (LC50 value > 105 µg/ml). Overall fourteen extracts were found to be non-toxic. Most herbal remedies were non cytotoxic but it would be necessary to complete these cyto-toxicological information by mutagenicity, teratogenicity tests as welle as in vivo toxicological tests on animals.


Keywords


Cytotoxicity; Medicinal plants; Cameroon; Pregnancy; Maternal-infant health

Full Text:

PDF

References


- Odukoya O. Traditional medicine practice in Nigeria: Targets and transformation quinta-feira. 2012. http://quintaisimortais.blogspot.com/2012.

- Zava DT, Dollbaum CM, Blen M. Estrogen and progestin bioactivity of foods, herbs and spices. SEBM 1998; 217: 369-371.

- Focho DA, Nkeng EAP, Lucha CF, Ndam WT, Afegenu IA. Ethnobotanical survey of plants used to treat diseases of the reproductive system and preliminary phytochemical screening of some species of malvaceae in Ndop Central Sub-division, Cameroon. J. Med. Plants Res. 2009; 3(4): 301-314.

- Yemele, MD, Telefo PB, Lienou LL, Tagne SR,. Fodouop CSP, Goka CS, Lemfack MC, Moundipa FP. Ethnobotanical survey of medicinal plants used for pregnant women's health conditions in Menoua division-West Cameroon. J. Ethnopharmacol. 2015; 160: 14–31.

- OMS (Organisation Mondiale de la Santé). Rapport sur la Santé dans le Monde 2005. Donnons sa chance à chaque mère et à chaque enfant. 2005 ; 68-69.

- UNICEF (Le Fonds des Nations Unies pour l’Enfance). La situation des enfants dans le monde 2009: La santé maternelle et néonatale. 2008 ; P4.

- Stuart D. Dangerous garden: the Quest For Plants to Change Our Lives. 2004.Havard University Press, Cambridge

- Deciga-Campos M, Rivero-Cry I, Arriaga-Alba M, Castaneda-Corral G, Angeles-Lopez GE, Navarrete A, Mata R. Acute toxicity and mutagenic activity of Mexican plants used in traditional medicine. J. Ethnopharmacol. 2007; 110: 334-337.

- Vajha M and Krishna CSR,. Evaluation of cytotoxicity in selected species of Caralluma and Boucerosia. Asian J. Plant Sci. Res., 2014; 4(4):44-47.

- Piccardi R, Frosini A, Tredici MR &Margheri MC. Bioactivity in free-living and symbiotic cyanobacteria of the genus Nostoc. J. Appl. Phycol. 2000; 12: 543-547.

- McLaughlin JL, Rogers LL., and Anderson JE. The use of biological assaysto evaluate botanicals. Drug Information Journal 1998; 32: 513–524.

- Meyer BN, Ferrigini RN, Jacobsen LB, Nicholas DE, Mc Laughlin JL Brine shrimp: a convenient general bioassay for active plant constituents. Planta Med 1982; 45:31-35.

- Rasoanaivo and Ratsimamanga-Urverg,. Biological Evaluation of Plants with reference to the Malagasy Flora. Napreca, Madagascar 1993: 9-43, 72-83.

- Zirihi GN, Mambu L, Guina FG, Bodo B, Grellier P. In vitro antiplasmodial activity and cytotoxicity of 33 West African plants used for treatment of malaria. J Ethnopharmacol. 2005; 98(3):281–285

- Eluwa MA, Udoaffah MT, Vulley MBG, Ekanem, potentials of crude ethanolic root bark and leaf extract of Rauwolfia vomitoria (apocynaceae) on the fetal heart. NAJMS 2010; 2(12): 592–595.

- Feutz A.C. Prenatal toxicity and teratogenicity: Principles, mechanisms and examples. 2013. http://ivf-basel.ch/uploads/media/Final_Prenatal_toxicity_and_teratogenesis_I_and_II_2013.pdf

- Sumalatha K.. Toxicity studies on ethanol extract of ageratum conyzoides l. in albino wistar rats. JPB 2012; 2(1): 7-11.

- Diallo K., Eklu-Gadegbeku, Amegbor K, Agbonon A, Aklikokou K, Creppy E, Gbeassor M. In vivo and in vitro toxicological evaluation of the hydroalcoholic leaf extract of Ageratum conyzoides L. (Asteraceae). J. Ethnopharmacol. 2014; 155(2): 1214–1218.

- Moshi MJ, van den Beukel CJP, Hamza OJM, Mbwambo ZH, Nondo ROS, Masimba PJ, Matee MIN, Kapingu MC, Frans, Verweij PE and van der Ven AJAM. Brine shrimp toxicity evaluation of some Tanzanian plants used traditionally for the treatment of fungal infections Afr J Tradit Complement Altern Med. 2007.4 (2): 219-225.

- Bum EN, Talla E, Dimo T, Sidiki N, Dawe A, Nguimbou RM, Dzeufiet PD, De Waard M. Evaluation of antinociceptive effects of Crassocephalum bauchiense Hutch (Asteraceae) leaf extract in rodents.J Ethnopharmacol. 2012;141 (1):234-241.

- Mouokeu RS, Ngono RAN, Tume C, Kamtchueng MO, Njateng GSS, Dzoyem JP, Tamokou JDD and Kuiate JR. Immunomodulatory Activity of Ethyl Acetate Extract and Fractions from Leaves of Crassocephalum bauchiense (Asteraceae). Pharmacologia, 2013; 4: 38-47;

- da Silva IF Jr, de Oliveira RG, Mendes SI, da Costa AT, Donizeti Ascêncio S, de Oliveira MDT. Evaluation of acute toxicity, antibacterial activity, and mode of action of the hydroethanolic extract of Piper umbellatum L. J Ethnopharmacol. 2014;151(1):137-43.

- Singh RK, Meena AK, Jain A and Pandey K. Acute Toxicity and Genotoxic Activity of Hibiscus rosa sinensis Flower Extract. AJPCT 2014; (2)(4)524-529.

- Singh SK, Prakash V, Toxicity assessment of oxalis corniculata and phyllanthus fraternusplants. Int J Pharm Pharm Sci 2014, 6(4): 388- 292.

- Tan PV,. Enow-Orock GE, Dimo T, Nyasse B,. Kimbu SF .Evaluation of the anti-ulcer and toxicity profile of aloe buettneri in laboratory animals. Afr J Tradit Complement Altern Med 2006; 3 (2)

- Oben JE, Assi SE, Agbor GA, Musoro DF. Effect of Eremomastax speciosa on experimental diarrhea. Afr J Tradit Complement Altern Med 2006 ; 3(1): 95-100.

- Dasgupta B, Kalita JC, Chowdhury A, Kotoky J. Hepatoprotective activity of Nelsonia canescens (Lam.) Spreng on acute hepatotoxicity induced by paracetamol. Int J Pharm Pharm Sci 2012; 4(1): 107-112.

- Telefo PB, Moundipa PF, Tchouanguep FM. Inductive effects of the leaf mixture extract of Aloe buettneri, Justicia insularis, Dicliptera verticillata and Hibicus macranthus on in vitro production of oestradiol. J. of Ethnopharmacol. 2004: 90, 225–230

- Ajayi IA, Ojelere OO.Chemical composition of ten medicinal plant seeds from Southwest Nigeria. ALST 2013; 10: 25-32.

- Galani YJH, Nguefack J, Dakole DC, Fotio D, Petchayo TS, Fouelefack FR, Amvam ZPH. Antifungal potential and phytochemical analysis of extracts from seven Cameroonian plants against late blight pathogen Phytophthora infestans. IJCMAS 2013; 2(5): 140-154

- Mouokeu RS, Ngono RAN, Lunga PK, Koanga MM, Tiabou AT, Njateng GSS, Tamokou JD, Kuiate JR. Antibacterial and dermal toxicological profiles of ethyl acetate extract from Crassocephalum bauchiense (Hutch.) Milne-Redh (Asteraceae). CAM 2011; 11:43.

- Dhole JA, Dhole NA, Lone KD, Bodke SS. Preliminary Phytochemical Analysis of Weeds in Marathwada Region. RJPBCS 2012; 3(4): 764-767.

- Badwaik H, Singh MK, Thakur D, Giri TK, Tripathi DK. The Botany, Chemistry, Pharmacological and Therapeutic Application of Oxalis Corniculata Linn. International Journal of Phytomedicine 2011; 3(1).

- Mensah JK, Okoli RI, Ohaju-Obodo JO, Eifediyi K,. Phytochemical, nutritional and medical properties of some leafy vegetables consumed by Edo people of Nigeria. AJB 2008; 7 (14): 2304-2309.


Refbacks

  • There are currently no refbacks.
';



Copyright (c) 2015 International Journal of Phytomedicine

                AR Journals

18K, Street 1st, Gaytri Vihar, Pinto Park, Gwalior, M.P. India

              Copyright@arjournals.org (Design) 2009-2020

 

Follow @arjournals on Twitter