Comparative study of methanolic and aqueous extracts of Cocculus hirsutus leaves on specific and non specific immune responses

Arunabha Mallik, Satish Nayak

Abstract


The aim of the present study was to find out comparatively about methanolic and aqueous extract of Cocculus hirsutus on specific and non specific immune responses in experimental mice. Oral administration of the methanolic  (200 and 400 mg/kg) and aqueous extract (250 and 500 mg/kg) were studied on various immune paradigm like determination of antibody titer, delayed type hypersensitivity reaction using SRBC as an antigen, carbon clearance assay as a measure of phagocytic index and total leukocyte count in cyclophosphamide induce immunosuppresed animals. Methanolic extract of Cocculus hirsutus to a large extent enhanced specific and non-specific activity in dose 200 and 400 mg/kg body weight. Aqueous extract at 250 mg/kg dose level failed to show appreciably immunomodulatory activity but 500 mg/kg of aqueous extract potentiated the activity however less significantly compared with both dose of methanolic extract.


Keywords


Immunomodulator, Cocculus hirsutus , Humoral immune responses, Delayed type hypersensitivity reaction, Phagocytosis

Full Text:

PDF

References


Kumar S, Gupta P, Sharma S, Kumar D. A review on immunostimulatory plants. J Chin Integr Med. 2011; 9(2): 117-128

Patwardhan B, Kalbagh, D, Patki PS, Nagsampagi BA. Search of immunomodulatory agents: a review. Indian Drugs. 1990; 28: 56–63.

Shivaprasad HN, Kharya MD, Rana AC, Mohan S. Preliminary immunomodulatory activities of aqueous extract of Terminalia chebula. Pharmaceutical Biology. 2006; 44: 32–34.

Puri A, Sahai A, Singh KL, Saxena RP, Tandon, Saxena KC. Immunostimulant activity of dry fruits and plant materials used in Indian traditional medical system for mothers after child birth and invalids. Journal of Ethnopharmacology. 2000; 71: 89 - 92.

Atal CK, Sharma ML, Kaul A, Khajuria A. Immunomodulating agents of plant origin. I: preliminary screening. Journal of Ethnopharmacology.1986; 18(2): 133-141.

Chopra RN, Chopra IC, Handa KL. Indigenous Drugs of India, U.N. Dhur & Sons Pvt Ltd., Calcutta.1958; pp. 501.

Nayak SK, Singhai AK. Anti-inflammatory and analgesic activity of roots of Cocculus hirsutus. Ind J Nat Prod. 1993; 9: 12-4.

Rao KV, Ramachandra RL. Chemical examination of Cocculus hirsutus (Linn) Diels. J Sci Ind Res. 1961; 20(B): 125-126.

Trease G.E, Evans MC. Textbook of Pharmacognosy, 12th ed. Balliere, Tindall, London. 1983; pp. 343–383.

Thakur M, Bhargava S, Dixit VK. Immunomodulatory activity of Chlorophytum borivilianum Sant. & F. Evidence Based Complementary and Alternative Medicine. 2007; 4(4): 419-423.

Singh S, Yadav CPS, Malleshappa N. Immunomodulatory activity of butanol fraction of Gentiana olivieri Griseb. On Balb/C mice. Asian Pac J Trop Biomed.2012; 2(6): 433-437.

Shinde UA, Phadke AS, Nair AM, Mungantiwar AA, Dikshit VJ, Saraf MN. Preliminary studies on the immunomodulatory activity of Cedrus deodara wood oil. Fitoterapia.1999; 70: 333-339.

Gokhale AB, Damre AS, Saraf MN. Investigations into the immunomodulatory activity of Argyreia speciosa. Journal of Ethnopharmacology.2003; 84: 109-114.

Shukla S, Mehta A, Johna J, Mehta P. Immunomodulatory activities of the ethanolic extract of Caesalpinia bonducella seeds. Journal of Ethnopharmacology.2009; 125: 252–256.

Dhumal JS, Yele SU, Ghodekar SN. Evaluation of immunomodulatory activity of Vigna mungo (L) hepper. J Pharm Phytother. 2013; 2: 9 – 14.

Gautama G, Sahaa S, Banib S, Kaulb A, Mishra S, Patil D. Immunomodulatory activity of Asparagus racemosus on systemic Th1/Th2immunity: implications for immunoadjuvant potential. J. Ethnopharmacol.2009; 121: 241-247.

Yadav R, Kharya DM, Yadav N, Savadi R. Immunomodulatory potential of ethanol extract of Spilanthus acmella leaves. Int. J. Biol. Med. Res. 2011; 2(3): 631-635.

Bafna AR, Mishra SH. Immunomodulatory activity of Methanol Extract of Flower Heads of Sphaeranthus indicus Linn. Ars Pharm.2004; 45: 281-291.

Sultana R, Khanam S, Devi K. Evalution of Immunomodulatory activity of Solanum xanthocarpum fruits aqueous extract. Der Pharmacia Lettre. 2011;3(1) :247-253

Damre AS, Gokhale AB, Phadke AS, Kulkarni, KR, Saraf M. Studies on the immunomodulatory activity of flavonoidal fraction of Tephrosia purpurea. Fitoterapia. 2003; 749: 257-260.


Refbacks

  • There are currently no refbacks.
';



Copyright (c)

                AR Journals

18K, Street 1st, Gaytri Vihar, Pinto Park, Gwalior, M.P. India

              Copyright@arjournals.org (Design) 2009-2021

 

Follow @arjournals on Twitter