In vitro antimicrobial, antioxidant, haemolytic, thrombolytic activities and phytochemical analysis of Cipadessa bacifera leaves extracts

T Thirunavukarasu, K Santhana Lakshmi, M Tamilarasan, S Sivamani, D Sangeetha, TP Rajesh


Objective: To study the leaves of Cipadessa bacifera for their antimicrobial, antioxidant, haemolytic, thrombolytic activities and to perform phytochemical evaluation.

Methods: The three extracts (methanol, cyclohexane, chloroform) of Cipadessa bacifera were screened for antimicrobial activity against eight pathogenic microorganisms by well diffusion method. In vitro antioxidant activity of extract was studied using hydrogen peroxide radical scavenging assay. The haemolytic activity was determined using agar diffusion techniques on blood agar plate, thrombolytic activity by clot disruption and phytochemical potential by qualitative analysis.

Results: Among the different extracts tested, the methanol extract of leaves showed significant antimicrobial activities. The most susceptible micro-organisms were found to be Gram negative bacteria (Sphingomonas, Klebsiella pneumoniae, Citrobacter), Gram positive bacteria (Enterococcus faecalis) and fungai (Candida albicans). H2O2 scavenging activity of Cipadessa bacifera was found to increase with increasing concentration of the extract. IC50 values of H2O2 scavenging activity was 100.92±0.41 µg/mL which was found in cyclohexane extract.  The haemolytic activity was found to be higher in chloroform extract than methanol, cyclohexane and the methanol extract shows 14.63 % clot lytic whereas standard streptokinase shows 30.86 % clot lytic activity in thrombolytic assay. The phytochemical evaluation indicates the presence of chemical constituents.

Conclusions: This study shows that the methanol and chloroform extract of leaves of Cipadessa bacifera has bioactivity but further compound isolation is necessary to confirm the activities of individual compound.


Antimicrobial activity, antioxidant, phytochemical evaluation, thrombolysis activity, haemolysis

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Borchers AT, Hackman RM, Keen CL, Stern JS, Gershwin ME. Complementary medicine: a review of immunomodulatory effects of Chinese herbal medicines. Am J Clin Nutr. 1997; 66(6):1303-12.

Cragg GM, Newman DJ. Biodiversity: A continuing source of novel drug leads. Pure Appl Chem. 2005;77:7-24.

Cowan MM. Plant products as anti-microbialagents. Clin Microbiol Rev. 1999;12:564-82.

Dahanukar SA, Kulkarni RA, Rege NN. Pharmacology of medicinal plants and natural products. Indian J Pharmacol. 2000;32:S81–S118.

Harborne JB. Phytochemical methods: A guide to modern techniques of plant analysis. Chapman and Hall. Newyork: 1973.

Kokate CK. Practical Pharmacognosy. Delhi: New Gyan Offset Printers; 2000.

Khandelwal KR. Practical Pharmacognosy. Pune: Nirali Prakashan. 2nd ed. 2009.

Kumar A, Ilavarasan R, Jayachandran, Decaraman M, Aravindhan P. Phytochemicals Investigation on a tropical plant in South India. Pak J Nutr. 2009;8:83-5.

McCracken WA, Cowsan RA. Clinical and Oral Microbiology. New York: Hemispher Publishing Corporation; 1983.

Ruch RJ, Cheng SJ, Klaunig JE. Prevention of cytotoxicity and inhibition of intracellular communication by antioxidant catechins isolated from Chinese green tea. Carcinogenesis. 1989;10:1003-1008.

Ahmed F, Islam MA, Rahman MM. Antimicrobial activity of Leonurus sibiricus aerial parts. FITOTERAPIA. 2006;77:316-317.

Pourmoard F, Hosseinimehr SJ, Shahabimajd V. Antioxidant activity, phenol and flavonoid contents of some selected Indian medicinal plants. Asian J Biotech. 2006;6:1197-201.

Gyamfi MA, Yonamine M, Aniya Y. Free radical scavenging action of medicinal herbs from Ghana Thonningla sanguine on experimentally induced liver injuries. General Pharmacol. 1999;32:661- 67.

Buhler DR and Miranda C. A net article: Antioxidant activities of Flavonoids, Department of Environmental and Molecular Toxicology Oregon State University. 2000.

Gillman MW, Cupples LA, Gagnon D, Posner BM, Ellison RC, Castelli WP, Wolf PA. Protective effect of fruits and vegetables on development of stroke in men. JAMA 1995;273:1113-1117.

Joshipura KJ, Ascherio A, Manson JE, Stampher MJ, Rimm EB, Speizer FE. Fruit and vegetable intake in relation to risk of ischemic stroke. JAMA 1999;282:1233-39.

Liu S, Manson JE, Lee I-M, Cole SR, Hennekens CH, Willett WC, Buring JE. Fruit and vegetable intake and risk of cardiovascular disease: the Women’s Health Study. Am J Clin Nutr. 2000;72:922-28.

Bazzano LA, He J, Ogden LG, Loria CM, Vupputuri S, Myers L, Whelton PK. Fruit and vegetable intake and risk of cardiovascular disease in US adults: the first National Health and Nutrition Examination Survey Epidemiologic Follow-up Study. Am J Clin Nutr. 2002;76:93-99.

Gallus AS. Thrombolytic therapy for venous thrombosis & pulmonary embolism. Bailliere Clin Haem. 1998;11:663-73.

Verstraete M. Third generation thrombolytic drugs. Am J Med. 2000;109:52-58.

Wardlaw JM, Berge E, del Zoppo G, Yamaguchi T. Thrombolysis for acute ischemic stroke. Stroke. 2004;35:2914-15.

Capstick T, Henry MT. Efficacy of thrombolytic agents in the treatment of pulmonary embolism. Eur Respir J. 2005;26:864-74.



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