Solubility Study and Partition Coefficients of 30% Ethanolic Extract Derived from Phyllanthus urinaria and Phyllanthus niruri between Newly Introduced Palm Kernel Oil Esters and Aqueous Vehicles

Elrashid Saleh Mahsi, Azmin Mohd Noor, Munavvar Abdul Sattar

Abstract


The purpose of this research was to study of physicochemical properties of the extracts and to develop and validate HPLC method in order to study their solubility in organic and aqueous vehicles and partition coefficients. The physicochemical characteristics such as moisture content, bulk density and were measured using various techniques. HPLC procedure for quantification of ellagic acid in the extracts has been modified and validated using binary gradient mode. A 0.2% formic acid and methanol was used to elute ellagic acid reference standard and the extracts on C 18 Hypersil Gold column stationary phase. The HPLC method was validated with respect to, linearity, system suitability, LOD, LOQ, accuracy and precision. The bulk density and moisture content results show of the extracts were varied, while the pH of the two extracts was almost similar. Palm kernel oil esters was solubilised extracts better  and Phosphate buffer at pH 7.4 and pH 6.6 were better aqueous vehicle for Phyllanthus urinaria and Phyllanthus niruri respectively and the extracts were partitioning better between phosphate buffer at pH 7.4 and  palm kernel oil esters. The HPLC method was simple, rapid, accurate and safe for the column and successfully applied to pre-formulation study of the extracts as skin cosmetics and palm kernel oil esters / phosphate buffer at pH 7.4 were chosen as oil and aqueous phase to formulate the extracts as skin cosmetics


Keywords


Solubility, Partition coefficients, Ellagic acid, Phyllanthus, Palm kernel oil esters

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References


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