Preparation of cyclodextrin nanoparticles and evaluation of its effect on the capacitation of bovine spermatozoa used in the in vitro fertilization

Heba F Salem, Mai Raslan, Hanaa Suliman, Tamer Essam, Saber Abd-Allah



This study was conducted to produce nanosized cyclodextrin (NCD) and assess its effect on bovine spermatozoa during In vitro fertilization (IVF) to optimize the capacitation media for successful IVF. Therefore, Four cyclodextrin formulations were prepared and characterized. Data analysis revealed the best formula (F2) showed a smallest particle size (15 nm), zeta potential (-37 mv), and higher yield percentages (95%) was selected for spem capacitation. Motile spermatozoa were separated from frozen-thawed semen by a swim-up procedure and capacitated in IVF-TALP medium with different formulae of NCD or CD or without treatments (control) and incubated for 3hours(hr) at 38°C and evaluated every one (hr) interval. Data analysis revealed that the formulation of cyclodextrin nanoparticles (F2) after (2hr) incubation in the media gave best effect on sperm capacitation and acrosme reaction (AR) and effect of sperm treated with NCD on fertilization rate was evaluated. The results showed that the proportion of Oocytes fertilized was increased significantly in F2 (60%) than in the control (35%), and cyclodextrin group (50%) groups (p<0.05). It could be inferred from this investigation that cyclodextrin nanoparticles can be used for biomedical interventions in bovine spermatozoa. NCD improve sperm motility, viability, and (AR), also fertilization rate of sperm treated with NCD increase. So NCD gave positive effect on sperm functions during IVF. 


Capacitaion; cyclodextrin; nanoparticles; In Vitro Fertilization; spermatozoa.


Horikoshi S, Serpone N. Microwaves in nanoparticle synthesis: fundamentals and applications: John Wiley & Sons; 2013.

[2]. Farokhzad OC, Langer R. Nanomedicine: developing smarter therapeutic and diagnostic modalities. Advanced drug delivery reviews. 2006;58(14):1456-9.

[3]. Yanagimachi R. Mammalian fertilization. The physiology of reproduction. 1994:189-317.

[4]. Lenz R, Ball G, Lohse J, First N, Ax R. Chondroitin sulfate facilitates an acrosome reaction in bovine spermatozoa as evidenced by light microscopy, electron microscopy and in vitro fertilization. Biology of reproduction. 1983;28(3):683-90.

[5]. Kitiyanant Y, Chaisalee B, Pavasuthipaisit K. Evaluation of the acrosome reaction and viability in buffalo spermatozoa using two staining methods: the effects of heparin and calcium ionophore A23187. International journal of Andrology. 2002;25(4):215-22.

[6].SaengerW. Cyclodextrininclusion compounds in research and industry. Angewandte Chemie International Edition. 1980;19(5):344-62.

[7]. Preaubert L, Courbiere B, Achard V, Tassistro V, Greco F, Orsiere T, et al. Cerium dioxide nanoparticles affect in vitro fertilization in mice. Nanotoxicology. 2016;10(1):111-7.

[8]. Nguyen T-D, Hong-Ngan Tran T, Nguyen C-H, Im C, Dang C-H. Synthesis and Characterization of β-Cyclodextrin/alginate Nanoparticle as a Novel Drug Delivery System. Chemical and biochemical engineering quarterly. 2015;29(3):429-35.

[9].Fujinoki M, Suzuki T, Takayama T, Shibahara H, Ohtake H. Profiling of proteins phosphorylated or dephosphorylated during hyperactivation via activation on hamster spermatozoa. Reproductive Medicine and Biology. 2006;5(2):123-35.

[10]. Larson JL, Miller DJ. (1999) Simple histochemical stain for acrosomes on sperm from33. Larson JL, Miller DJ. Simple histochemical stain for acrosomes on sperm from several species. Molecular reproduction and development. 1999;52(4):445-9.

[11]. Abd-Allah SM. Successful cryopreservation of buffalo ovaries using in situ oocyte cryopreservation. Vet Ital. 2009;45(4):507-12.

[12]. Goodrowe K, Wall R, O’Brien SJ, Schmidt P, Wildt DE. Developmental competence of domestic cat follicular oocytes after fertilization in vitro. Biology of reproduction. 1988;39(2):355-72.

[13]. Zerkoune L, Angelova A, Lesieur S. Nano-assemblies of modified cyclodextrins and their complexes with guest molecules: Incorporation in nanostructured membranes and amphiphile nanoarchitectonics design. Nanomaterials. 2014;4(3):741-65.

[14]. Cheng F, Fazeli A, Voorhout W, Tremoleda J, Bevers M, Colenbrander B. Progesterone in mare follicular fluid induces the acrosome reaction in stallion spermatozoa and enhances in vitro binding to the zona pellucida. International journal of andrology. 1998;21(2):57-66.

[15]. Meyers SA, Overstreet JW, Liu IK, Drobnis EZ. Capacitation In Vitro of Stallion Spermatozoa: Comparison of Progesterone‐Induced Acrosome Reactions in Fertile and Subfertile Males. Journal of andrology. 1995;16(1):47-54.

[16]. Rathi R, Colenbrander B, Stout T, Bevers M, Gadella B. Progesterone induces acrosome reaction in stallion spermatozoa via a protein tyrosine kinase dependent pathway. Molecular reproduction and development. 2003;64(1):120-8.

[17]. Parrish J, Susko-Parrish J, Winer M, First N. Capacitation of bovine sperm by heparin. Biology of reproduction. 1988;38(5):1171-80.

[18].Thérien I, Manjunath P. Effect of progesterone on bovine sperm capacitation and acrosome reaction. Biology of reproduction. 2003;69(4):1408-15.

[19]. Nassar A, Mahony M, Morshedi M, Lin M-H, Srisombut C, Oehninger S. Modulation of sperm tail protein tyrosine phosphorylation by pentoxifylline and its correlation with hyperactivated motility.fertility and Sterility.1999;71(5):919-23.


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