Nanoparticle preparation and characterization of Haruan fish (Channa striata) exctract contains albumin from south Kalimantan with ionic gelation method

Rahmawanty D, Risa A, Malikhatun N, Prima HR, Nani K, Effionora A

DOI: http://dx.doi.org/10.5138/09750215.2070

Abstract


Snakehead fish (Channa striata) has been reported to be used for wound healing by people in South Borneo because it contains albumin. Snakehead fish extract (Channa striata) has hydrophillic property and poor stability. Nanoparticle technology has been started to be developed as an alternative solution to improve drug delivery profile. The purpose of this study was to determine the formulation that obtained best characterization for nanoparticle. Nanoparticles were prepared by ionic gelation method, that was prepared by doing optimize ratio between snakehead fish extract : chitosan and pH of chitosan solvent.Nanoparticles were characterized using Particle Size Analyzer for particle size and particle size distribution, measurement of entrapment efficiency, determined Zeta potential using Particle Size Analyzer, and observation of particle’s morphology using Transmission Electron Microscope. The result showed that the chosen formula was formula 6 which  ratio of extract : chitosan 1:2 with chitosan solvent pH 3, particle size 152.3 nm, polidispersity index 0.778, percentage of entrapment efficiency 51.3961 %, Zeta potential +35.9 mV, and round shape of particles.


Keywords


ionic gelation, nanoparticle, snakehead fish (Channa striata)

Full Text:

PDF

References


[1]. Burnawi. Identifikasi Jenis Ikan di Suaka Perikanan Awang Landas Sungai Barito, Kalimantan Selatan. Buletin Teknik Litkayasa. 2012;10 (1): 27-30.

[2]. Cahyana SPD. Ikan Gabus di Kancah Nasional. Badan Litbang Pertanian. Banjarmasin 2013.

[3]. Tiyaboonchai W. Chitosan Nanoparticles : A Promising System for Drug Delivery. Naraseuan University Journal. 2003;11 (3): 51-66.

[4]. Lohcharoenkal W, Wang L, Chen YC & Rojanasakul Y. Protein Nanoparticles as Drug Delivery Carriers for Cancer Therapy: Review Article. BioMed Research International. Volume 2014, Article ID 180549.

[5]. Sinko PJ & Singh Y. Martin’s Physical Pharmacy and Pharmaceutical Science Sixth Edition. Lippincott Williams & Wilkins 2011.

[6]. Iswandana R. Preparasi Nanogel Verapamil Hidroklorida Menggunakan Metode Gelasi Ionik antara Kitosan-Natrium Tripolifosfat sebagai Sediaan Antihipertensi. Tesis, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Indonesia. Depok 2012.

[7]. Kafshgari MH, Khorram M, Khodadoost M & Khavari S. Reinforcement of Chitosan Nanoparticle Obtained by an Ionic Cross-Linking Process. Irian Polymer Journal. 2011;20 (5): 445-456.

[8]. Bahreini Bradford MM. A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding. Journal of Analytical Biochemistry. 1976;72:248-254.

[9]. Asfar M, Tawali AB, Abdullah N. & Mahendradatta M. Extraction of Albumin of Snakehead Fish (Channa striatus) in Producing The Fish Protein Concentrate (FPC). International Journal of Scientific & Technology Research. 2014;3 (4): 85-88.

[10]. Daud CKD, Jais AMM, Ahmad Z, Akim AMD & Adam A. Amino and Fatty Acid Compositions in Haruan Traditional Extract (HTE). Boletín Latinoamericano y del Caribe de Plantas Medicinales y Aromática. 2010; 9 (5): 414 -429.

[11]. Sivasankar M & Kumar B. Role of Nanoparticles in Drug Delivery System. International Journal of Research in Pharmaceutical and Biomedical Sciences. 2010;1 (2).

[12]. Gam LH, Leow CY & Baie S. Proteomic Analyisis of Snakeheadfish (Channa striata) Muscle Tissue. Malaysian Journal of Biochemistry and Molecular Biology. 2006;14:25-32.

[13]. Nurkhasanah T, Yuwono M, Rizki I & Kraisintu K. The Development of Chitosan Nanoparticles from Hibiscus Sabdariffa L. Calyx from Indonesia and Thailand. International Journal of Pharmaceutical Science and Research. 2015;6 (5): 1855-1861.

[14]. Rizki MI. Formulasi Nanopartikel Kitosan Ekstrak Bunga Rosella (Hibiscus sabdariffa) dan Uji Aktivitas Antioksidan pada Sel Darah Merah Domba. Tesis, Fakultas Farmasi, Universitas Ahmad Dahlan. Yogyakarta 2014.

[15]. Honary S & Zahir F. Effect of Zeta Potential on the Properties of Nano-Drug Delivery Systems -A Review (Part 1). Tropical Journal of Pharmaceutical Research. 2013;12 (2): 255-264

[16]. Martien R, Adyatmika ID, Irianto K, Farida V, dan D, Sari P. Perkembangan Teknologi Nanopartikel sebagai Sistem Penghantaran Obat. Majalah Farmaseutik. 2012;8 (1): 133-144.

[17]. Liu H & Gao C. Preparation and Properties of Ionically Cross-linked Chitosan Nanoparticles. Polym. Adv. Technol. 2009; 20 613–619.


Refbacks

  • There are currently no refbacks.




Copyright (c) 2017 dina rahmawanty

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Advanced Research Journals

18K, Street 1st, Gaytri Vihar, Pinto Park, Gwalior, M.P. India

Copyright@arjournals.org (Design) 2009-2016

 

Follow @arjournals on Twitter